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To generate bacterial expression constructs of VV His-A51R and its mutants, the A51R ORF was PCR-amplified from FA51R pCDNA3 vectors using primers encoding an N-terminal His-tag and NcoI/NotI cut sites and the resulting products were ligated into a modified pET22b vector in which the pelB sequence was removed

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Our Advanced Medical Care team can also administer glutathione directly into your bloodstream with a simple injection or IV drip