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Sample preparation for T cell proteomics A375 melanoma tumor cells (ATCC, CRL-1619) were cultured in complete DMEM (Gibco, 11965-092) supplemented with 10% (v/v) FBS (VWR, 97068-085) and 1% penicillin–streptomycin (VWR, 16777-164) at 37 °C under 5% CO 2
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Principal component analysis (PCA) was used for exploratory visualization of global transcriptomic variability, and group differences were assessed using permutational multivariate analysis of variance (PERMANOVA)
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