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6 Tools and techniques for assessing ENM-Microbiome interactions Assessing the impact of ENMs on microbiome interactions requires a comprehensive, multi-tiered approach that integrates high-throughput sequencing (16S rRNA, metagenomics, metatranscriptomics), functional assays (enzyme activity, SCFA profiling, metaproteomics, metabolomics), resistome analysis (qPCR, metagenomics), microscopy techniques (TEM, confocal), and in vitro / in vivo models (organoids and organ-on-a-chip technologies, gnotobiotic mice, “Humanized” murine models) to evaluate compositional, functional, and genetic changes

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